Study of the Effect of Green Nanocomposite from Salvia officinalis Plant on pslA Gene in Ciprofloxacin-resistant Pseudomonas aeruginosa Bacteria

The current study aimed to get ready aqueous extract and nanocomposite of the Salvia officinalis plant using the green synthesis method, and to identify silver nanoparticles, an AFM microscope was used, the antibiotic was loaded onto the nanocomposite, and the genetic expression of the psla gene was studied in the Pseudomonas aeruginosa bacteria. The study groups included five groups, the first group was treated with the antibiotic. Ciprofloxacin, the second group was treated with the aqueous extract of the Salvia officinalis plant, the third group was treated with the nanocomposite, the fourth group was treated with the extract loaded with the antibiotic, and the fifth group was treated with the nanocomposite loaded with the antibiotic. The results showed that change in color from yellow to dark brown Indicates the presence of nanoparticles and that the average size average granular size of the extract loaded with the antibiotic is 132.2nm, while the average granular size of the free extract is 85.49nm, and the average granular size of the nanocomposite loaded with the antibiotic is 92.25nm, while the average granular size of the free nanocomposite is 35.42nm, which indicates the success of the process of loading the antibiotic ciprofloxacin onto the nanocomposites, and down-regulation occurred in Gene expression of the psla gene in P. aeruginosa. In the fourth treatment, the aqueous extract of Salvia officinalis is loaded with the antibiotic by (0.8643±0.0095 fold change) and in the fifth treatment, the nanocomposite is loaded with the antibiotic by (0.4357±0.0088 fold change) when the concentration is greater than the MIC.