A Study Protocol for Evaluation of Microcytic Hypochromic Anemia by High-Performance Liquid Chromatography (HPLC)

Background: One of the most regularassessments done in clinical practice is anemia. Different levels classify the anemiasbased onthe numerical value of hemoglobin, alteration in the morphology and chromium, underlying etiologies, red cell volumetric parameters and functionally depending on pathophysiologic processes of anemia. Microcytic hypochromic anemia (MCHC) has different underlying causes, including iron deficiency anemia, beta-thalassemia trait, and hemoglobinopathies. Before planning the treatment, assessing the hemoglobin for its variants and the detection of abnormal hemoglobinis mandatory. High-performance liquid chromatography (HPLC) has proved to be significant for the evaluation of MCHC because of its superior separation and quantification analytical powers.The present study has been undertakingthat anemia is the most prevailing and commonly treated clinical state managed by numerous nutritional supplements without much being done to know the underlying etiology or without evaluation of hemoglobin for its abnormalities.

Methods: This will bean observational (Prospective and Retrospective) study. Blood samples of 100 patients will be evaluated by HPLCBio-Rad variant,and various hemoglobin patterns; associate dhemoglobinopathies will be evaluated.

Results: The observations will be made according to the objectives and tabulated, which will be subjected to statistical tests for their significance and conclusions.

Conclusion: This study would contribute to understanding and knowing the etiology of MCHC in the population that our tertiary care hospitals serve.