Resistin Inhibits the Synthesis of Insulin by miR-494 through Traget on CREB1

Resistin was first described as an adipokine and found to impair the pancreatic beta cells. The reason that it was named “resistin” is because it related with insulin resistance. Our previous study showed that miR-494 were significantly downregulated by resistin, while it is unknown that whether miR-494 involved in the regulation of insulin synthesis induced by resistin. The current study investigated the effects of miR-494 in the insulin synthesis reduced by resistin. MIN6 cells were treated with resistin and insulin synthesis was measured, the results showed that the insulin synthesis were significantly reduced by resistin. The over-expression of miR-494 inhibited the insulin synthesis both in diet culture and high glucose medium. Further, we discovered that miR-494 down-regulated the protein level of CREB1 by pairing with sites in the 3’UTR, which suggested that CREB1 is one of a target genes of miR-494. In conclusion, resistin inhibit the synthesis partly by miR-494 through target on CREB1 gene.