Effect of Standardized Polyherbal Formulations on Blood Glucose Body Weight Food and Water Consumption of Rats

Standardization of polyherbal formulation is mandatory to evaluate the best quality of drugs for therapeutic value. The main objective of this study was to develop a standardized, safe and effective polyherbal juice with significant anti-oxidant and anti-diabetic activity. Methods: Polyherbal juice was formulated using cold maceration method and was evaluated for Total phenolic content, Total flavonoid content, Antioxidant activity, Particle size, IR, HPTLC and Heavy metal analysis. Results: Total phenolic content was found to be 139.23 ± 0.072 mg/g gallic acid equivalent and Total flavonoid content was 62.01± 0.063 mg/g Catechin equivalent. In DPPH scavenging assay the RC50 value of polyherbal formulation was found to be 6.0 µg/ml. Preliminary phytochemical tests and HPTLC was performed for Quantitative Analysis of Phytoconstituents in formulation. Conclusion: In-vivo study shows significant anti-diabetic activity as compared with marketed formulations. Thus polyherbal formulation possesses good anti-diabetic and antioxidant activity.